A Method for Preparing a Permanent Nocht's Stain (nocht-jenner Stain)

The possibility of preparing a permanent staining solution from the formulas for Nocht's (I) stain was suggested by the constant use of Jenner's (2) stain for routine blood examinations and malarial staining during the summer and fall months of ~9ox. In malarial blood the pigmented bodies of tertian fever and the crescent bodies of mstivo-autumnal fever are readily demonstrated with Jenner's stain, and the use of poly-chrome stains was resorted to only in doubtful cases showing no full-grown forms of parasites. Goldhorn's (3) method of staining with the polychrome blue prepared from methylene blue and lithium carbonate proved valuable, but we were never able to obtain the constantly per-feet results obtained by employing the methods of Noeht as described by Lazier (4) and Ewing (5). No other method gives results comparable to these. The only objection to them is the time required (from two to twelve hours) for good staining. During the fall of ~9oI, repeated attempts to prepare a permanent staining fluid by Nocht's method succeeded in so far that in the later preparations the same method of procedure was followed, but we were unable to obtain the polychrome tint for the nuclear material and the chromatin staining in the malarial parasite until the publication of Leishman (6) suggested the final simple step in staining, that of diluting the staining fluid with distilled water after staining one minute in full strength and allowing this diluted stain to remain in contact with the specimen from three to five minutes.